Characterization and location of secretory phospholipase A2 groups IIE, V, and X in the rat brain

✍ Scribed by Miriam Kolko; Nanna R. Christoffersen; Sebastian G. Barreiro; Martin L. Miller; Andrew J. Pizza; Nicolas G. Bazan

• Book ID: 102908820
• Publisher: John Wiley and Sons
• Year: 2006
• Tongue: English
• Weight: 799 KB
• Volume: 83
• Category: Article
• ISSN: 0360-4012
• DOI: 10.1002/jnr.20773

✦ Synopsis

Abstract

Secretory phospholipases A~2~ (sPLA~2~) form a diverse family of enzymes involved in a variety of physiologic and pathologic processes. Common among all sPLA~2~ is the ability to cleave the second position of phospholipids, thereby releasing fatty acid and a lysophospholipid. Several sPLA~2~ have been cloned and characterized in various tissues and receptors have been identified. In the nervous system, sPLA~2~ groups IB, IIA, IIE, IIF, V, and XII have been identified, and binding sites for sPLA~2~ have been found. Here, we report sPLA~2~‐IIE and sPLA~2~‐X in rat brain as well as in neurons in primary culture. We furthermore confirm the presence of sPLA~2~‐V in rat brain and demonstrate the presence of sPLA~2~‐V in primary neuronal cultures. The distribution of sPLA~2~‐IIE, V, and ‐X seems to be mainly neuronal, with the highest abundance occurring in the cerebral cortex and hippocampus. We also find that sPLA~2~‐IIE, ‐V, and ‐X are differentially induced by kainic acid. This study supports the concept that sPLA~2~ heterogeneity in brain is functionally relevant and responsive to seizures. © 2006 Wiley‐Liss, Inc.