Characterization and enrichment of fetal rat hepatoblasts by immunoadsorption (“panning”) and fluorescence-activated cell sorting

✍ Scribed by Samuel H. Sigal; Shlomo Brill; Lola M. Reid; Isabel Zvibel; Sanjeev Gupta; Douglas Hixson; Ronald Faris; Patricia A. Holst

• Publisher: John Wiley and Sons
• Year: 1994
• Tongue: English
• Weight: 1023 KB
• Volume: 19
• Category: Article
• ISSN: 0270-9139
• DOI: 10.1002/hep.1840190427

✦ Synopsis

We developed methods for enriching fetal hepatoblasts by combining panning and rnultiparametric fluoremame-activated cell sorting. In unpurifid, dissociated fetal liver cell suspensions of embryonic age day 16, 3.2% f 1.3% and 2.6% * 0.7% cells expressed albumin and a-fetoprotein, respectively. The remainder d b i t e d a hemopoietic, endothelial or stromal cell phenotype. Cells were panned rirst with an antibody to red blood cells to remove erythroid cells and then with monoclonal antibodies OX-43/OX-44 to remove hemopofetic and endothelial cells. This procedpre eliminated 84% of fetal hepatic cells, with enrichment of the remainder for albumin or a-fetoprotein eqwemrion (up to sixfold increase). Flow cytometric agelygie of unlabeled cells revealed two populations, which differed in granularity and autofluoree-