High level expression and crystallizatio
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Dieter BrΓΆmme; Mary E. Mcgrath
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Article
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2008
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Cold Spring Harbor Laboratory Press
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English
β 274 KB
## Abstract We have expressed active human cathepsin S to 60 mg/L in Sf9 cells using a baculovirus system. Production of milligram quantities has facilitated crystallographic studies to determine the structure of this enzyme, which has unique properties among lysosomal cysteine proteinases. Recombi