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Characterization and application of the biotin-switch assay for the identification of S-nitrosated proteins

✍ Scribed by Yanhong Zhang; Agnes Keszler; Katarzyna A. Broniowska; Neil Hogg


Book ID
113629491
Publisher
Elsevier Science
Year
2005
Tongue
English
Weight
306 KB
Volume
38
Category
Article
ISSN
0891-5849

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Endogenous ligands complicate radioligand-binding assays of high-affinity binding proteins by obscuring binding sites or by diluting the labeled ligand. We have developed a mathematical model for such systems where radioligand and endogenous ligand are structurally identical. Data which relate radio