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Characterization and analysis of thermal denaturation of antibodies by size exclusion high-performance liquid chromatography with quadruple detection

✍ Scribed by Wanda K. Hartmann; Nirmala Saptharishi; Xiao Yi Yang; Gautam Mitra; Gopalan Soman


Book ID
108047623
Publisher
Elsevier Science
Year
2004
Tongue
English
Weight
393 KB
Volume
325
Category
Article
ISSN
0003-2697

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Collagen extracted from tissues by pepsin digestion is a mixture of monomeric and oligomeric molecules. The oligomers are held together by covalent crosslinks between molecules. A simple size-exclusion high-performance liquid chromatography (HPLC) method for separation of collagen monomers and oligo