Characteristics of the immobilized pectin lyase activity from a commercial pectolytic enzyme preparation
✍ Scribed by P. Lozano; A. Manjón; J. L. Iborra; D. M. Gálvez
- Publisher
- John Wiley and Sons
- Year
- 1990
- Tongue
- English
- Weight
- 477 KB
- Volume
- 10
- Category
- Article
- ISSN
- 0138-4988
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✦ Synopsis
A commercial pectolytic enzyme preparation has been immobilized onto a nylon-polyethyleneimine copolymer, in order to study the contribution of the pectin lyase activity in the overall pectindegrading activity. Optimal conditions for the immobilization process of the pectin lyase activity, such as chemical modification of the support, coupling pH and protein concentration, were determined. Kinetic parameters and temperature behaviour of both the soluble and immobilized pectin lyase activity of the derivative were also determined. Operational stability of the pectin lyase and overa l l viscosity reducing activities resulting in a halflife times of 3.8 and 8.5 days, respectively, for both pectolytic activities, when the immobilized derivatives were tested in a cross-flow reactor, using a highly esterificd pectin as substrate.