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Characteristics of sodium-induced increase in opiate antagonist binding sites in rat brain membranes

โœ Scribed by Peter C.G. Nijssen; Steven R. Childers


Book ID
115881086
Publisher
Elsevier Science
Year
1987
Tongue
English
Weight
793 KB
Volume
135
Category
Article
ISSN
0014-2999

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The sulfhydryl alkylating reagent N-ethylmaleimide (NEM) blocks opioid receptor binding and receptor/G-protein coupling. Sodium partially restores [ 3 H]naloxone binding after inhibition by NEM to reveal sodium-dependent [ 3 H]naloxone sites, defined as binding in the presence of 50-100 mM NaCl afte