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Characteristics of protein tyrosine kinase activities of Y-79 retinoblastoma cells and retina

โœ Scribed by Susan Gentleman; Paul Russell; Todd M. Martensen; Gerald J. Chader


Book ID
115706804
Publisher
Elsevier Science
Year
1985
Tongue
English
Weight
544 KB
Volume
239
Category
Article
ISSN
0003-9861

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We report a simple method that permits simultaneous detection of multiple protein kinase activities using postnuclear supernatant of \(\mathbf{v}-8\) src transformed NIH3T3 cells. A supernatant is incubated with activators of protein kinases and \(\left[\gamma-{ }^{32} \mathbf{P}\right] \mathrm{ATP}