Characteristics of immobilized lipase-catalyzed hydrolysis of olive oil of high concentration in reverse phase system
✍ Scribed by Sung Tae Kang; Joon Snick Rhee
- Publisher
- John Wiley and Sons
- Year
- 1989
- Tongue
- English
- Weight
- 770 KB
- Volume
- 33
- Category
- Article
- ISSN
- 0006-3592
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## Abstract Candida rugosa lipase has been used to investigate the hydrolysis of high concentration olive oil in the AOT–isooctane reversed micellar system at __W__~__o__~ = 10, pH 7.1, and 37°C. Results from this work show the hydrolytic reaction obeys Michaelis–Menten kinetics up to the initial s
Lipase (EC 3.1.1.3) from Candida rugosa was immobilized with DEAE-Sephadex A50, Sephadex G50, Sephadex LH-20, Amberlite IRA94, and Amberlite XAD-7. The enzyme immobilized with DEAE-Sephadex A50 was found to be most effective for continuous hydrolysis of olive oil in isooctane. For the continuous rea