Development of a Mechanism-Based, DNA St
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Xiaomei Yan; Robert C. Habbersett; Julia M. Cordek; John P. Nolan; Thomas M. Yos
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Article
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2000
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Elsevier Science
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English
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Accurate measurement of single DNA fragments by DNA fragment sizing flow cytometry (FSFC) depends upon precise, stoichiometric DNA staining by the intercalating dye molecules. In this study, we determined the binding characteristics of a commercially available 532 nm wavelength-excitable dye and use