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Characteristics of Different Nucleic Acid Staining Dyes for DNA Fragment Sizing by Flow Cytometry

โœ Scribed by Yan, Xiaomei; Grace, W. Kevin; Yoshida, Thomas M.; Habbersett, Robert C.; Velappan, Nileena; Jett, James H.; Keller, Richard A.; Marrone, Babetta L.


Book ID
125900167
Publisher
American Chemical Society
Year
1999
Tongue
English
Weight
159 KB
Volume
71
Category
Article
ISSN
0003-2700

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Accurate measurement of single DNA fragments by DNA fragment sizing flow cytometry (FSFC) depends upon precise, stoichiometric DNA staining by the intercalating dye molecules. In this study, we determined the binding characteristics of a commercially available 532 nm wavelength-excitable dye and use