Characteristics and isolation of the phagocytosis-stimulating peptide, tuftsin
β Scribed by Kenji Nishioka; Andreas Constantopoulos; Paul S. Satoh; William M. Mitchell; Victor A. Najjar
- Book ID
- 115749408
- Publisher
- Elsevier Science
- Year
- 1973
- Weight
- 750 KB
- Volume
- 310
- Category
- Article
- ISSN
- 0005-2795
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Incubation of human polymorphonuclear leukocytes (PMNL) or thioglycollate-stimulated mouse peritoneal macrophages with the phagocytosis-stimulating peptide, tuftsin (2.5 X 10(-7) M, at 37 degrees C), caused an increase of 80-90% in intracellular cGMP levels, accompanied by a decrease of 20-25% in in
## Abstract A radioimmunoassay was developed for the phagocytosisβstimulating peptide tuftsin, HβThrβLysβProβArgβOH, and preliminary results of its use in evaluating tuftsin levels in human blood are presented. Synthetic tuftsin was rendered antigenic through coupling of pβdiazonium phenylacetylβt