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Characteristics and isolation of the phagocytosis-stimulating peptide, tuftsin

✍ Scribed by Kenji Nishioka; Andreas Constantopoulos; Paul S. Satoh; William M. Mitchell; Victor A. Najjar


Book ID
115749408
Publisher
Elsevier Science
Year
1973
Weight
750 KB
Volume
310
Category
Article
ISSN
0005-2795

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Incubation of human polymorphonuclear leukocytes (PMNL) or thioglycollate-stimulated mouse peritoneal macrophages with the phagocytosis-stimulating peptide, tuftsin (2.5 X 10(-7) M, at 37 degrees C), caused an increase of 80-90% in intracellular cGMP levels, accompanied by a decrease of 20-25% in in

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## Abstract A radioimmunoassay was developed for the phagocytosis‐stimulating peptide tuftsin, H‐Thr‐Lys‐Pro‐Arg‐OH, and preliminary results of its use in evaluating tuftsin levels in human blood are presented. Synthetic tuftsin was rendered antigenic through coupling of p‐diazonium phenylacetyl‐t