Characteristic features of the sonicated DNA ofAgama agama agamaL. (Reptilia, Agamidae) on hydroxyapatite columns, using mouse DNA as a reference
✍ Scribed by J. A. Adegoke; B. O. Ighavini; R. O. Onuigbo
- Book ID
- 104631952
- Publisher
- Springer Netherlands
- Year
- 1991
- Tongue
- English
- Weight
- 758 KB
- Volume
- 83
- Category
- Article
- ISSN
- 0016-6707
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✦ Synopsis
Hydroxyapatite column chromatography has been used to study some properties of the extensively sheared DNA of the Rainbow lizard, Agama agama agama. Reassociation studies show that the genome has a Cot1/2 of 370. Approximately 15% of the genome is highly repetitive in nature. This repetitive fraction is resolved into thermally stable and less stable fractions. The stable fraction has a base composition of 47% GC, higher than the 40.2% GC for the native DNA. This stable fraction is believed to be of recent origin. Chromatography of the total DNA of the lizard with linear gradients of phosphate buffer containing 1 M urea resolves it into two components which were shown by thermal fractionation, also in the presence of 1 M urea, to vary in base composition. This behaviour may be characteristic of reptilian genomes and may be used as a basis for studying the structural organisation of the reptilian genome.