Characteristic differences in monosaccharide composition of glycoconjugates from opposite mating types of Chlamydomonas eugametos
✍ Scribed by Gerrit J. Gerwig; Johannis P. Kamerling; Johannes F.G. Vliegenthart; Wieger L. Homan; Piet van Egmond; Herman van den Ende
- Book ID
- 102641338
- Publisher
- Elsevier Science
- Year
- 1984
- Tongue
- English
- Weight
- 413 KB
- Volume
- 127
- Category
- Article
- ISSN
- 0008-6215
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✦ Synopsis
The monosaccharide composition of the isoagglutinins derived from the flagellar membranes of the opposite mating types of Chlamydomonas eugametos (mt+ and mt-) differ strikingly. Besides the common occurrence of L-arabinose, Lrhamnose, D-xylose, D-mannose, D-galactose, L-galactose, D-&COW, and 2acetamido-2-deoxy-D-glucose, the mtf isoagglutinin carbohydrate chains contain gulosc, 4-0methylxylose, a 2-0-methylpentose, and 3-0-methyl-D-galactose, whereas those of the mtisoagglutinin contain 6-0-methyl-D-mannose and 3-0methyl-L-glucose. Monosaccharide constituents were analysed by g.1.c. and g.l.c.m.s. of trimethylsilylated methyl glycosides and alditol acetates.
For the assignment of the absolute configurations using g.l.c., trimethylsilylated ( -)-2-butyl glycosides were analysed. In view of the absolute specificity of the agglutination process displayed by the isoagglutinins of the sexually different mating types, the carbohydrate differences might be significant for the sexual interaction.