A large number of opium alkaloids has been chromatographed by gas, thin-layer, and paper chromatography for the purpose of identification. Gas chromatography is particularly valuable because of its speed, sim licity, and reproducibility. T h e two other methods are useful for certain alkaloi& which
Characterisation of pyrrolizidine alkaloids by gas, thin-layer and paper chromatography
β Scribed by A.H. Chalmers; C.C.J. Culvenor; L.W. Smith
- Publisher
- Elsevier Science
- Year
- 1965
- Tongue
- English
- Weight
- 789 KB
- Volume
- 20
- Category
- Article
- ISSN
- 1873-3778
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The separation of twelve common Rauwolfia alkaloids for quantitative thinlayer chromatography can be effected using a minimum of five solvent systems on silica gel layers. The alkaloids can be identified by chromogenic reactions and ultraviolet and infrared spectrometry.
## Abstract Recently, contamination of honey with pyrrolizidine alkaloids (PA) has been reported as potential health risk. Therefore, it was of interest to develop a reliable tool for selective and quantitative determination of PA in honey. Sample preparation of the novel method comprises strong ca
produccs a curved segment a t lower pH values.8 In calculating the purity and ionization constant, only the linear portion of each plot was used. Nevertheless, it may be seen (Table 11) that the intercept obtained (and therefore the purity evaluated) is not markedly influenced by the choice of activ