✦ LIBER ✦

Characterisation of poly(ADP-Rib) polymerase activity in nuclei from the slime mould dictyostelium discoideum

✍ Scribed by D. Rickwood; M. S. Osman

Publisher: Springer
Year: 1979
Tongue: English
Weight: 397 KB
Volume: 27
Category: Article
ISSN: 0300-8177
DOI: 10.1007/bf00218351

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✦ Synopsis

Nuclei isolated from D. discoideum and incubated in vitro with 3H-NAD synthesise poly(ADP-Rib). The optimum incubation conditions for the poly(ADP-Rib) polymerase were determined. The Km of the enzyme is 18 microM NAD and it is inhibited by nicotinamide. Most of the poly(ADP-Rib) synthesised is attached to nuclear proteins.