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Characterisation of a salt-stimulated ATPase activity associated with vacuoles isolated from storage roots of red beet (Beta vulgarisL.)

✍ Scribed by Robert R. Walker; Roger A. Leigh

Publisher: Springer-Verlag
Year: 1981
Tongue: English
Weight: 970 KB
Volume: 153
Category: Article
ISSN: 0032-0935
DOI: 10.1007/bf00384095

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✦ Synopsis

Ion stimulation and some other properties of an ATPase activity associated with vacuoles isolated from storage roots of red beet (Beta vulgar& L.) have been determined. The ATPase had a specific requirement for Mg 2+ and in the presence of Mg 1+ it was stimulated by salts of monovalent cations. The degree of stimulation by monovalent salts was influenced mainly by the anion and the order of effectiveness of the anions tested was C1-> HCO~ > Br-> malate > acetate > SO42-. For any given series of anions the magnitude of the stimulation obtained was influenced by the accompanying cation (NH2>> Na+>K+). This cation effect was abolished by 0.01% (v/v) Triton X-100 and it is suggested that it is the result of different permeabilities of membrane vesicles to the cations. There was no evidence of synergistic stimulation of the ATPase by mixtures of Na + and K +. KC1-and NaCl-stimulation was maximal with salt concentrations in the range 60-150 mM. The true substrate of the enzyme was shown to be MgATP. It was shown that KC1 stimulation was the result of an increase in Vm~x rather than a change in the affinity of the enzyme for MgATP. The ATPase * Recipient of a C.

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