Characterisation by 1H NMR spectroscopy of oligosaccharides derived from alkali-extractable wheat-flour arabinoxylan by digestion with endo-(1 → 4)-β-d-xylanase III from Aspergillus awamori
✍ Scribed by Felix J.M. Kormelink; Rainer A. Hoffmann; Harry Gruppen; Alphons G.J. Voragen; Johannis P. Kamerling; Johannes F.G. Vliegenthart
- Publisher
- Elsevier Science
- Year
- 1993
- Tongue
- English
- Weight
- 745 KB
- Volume
- 249
- Category
- Article
- ISSN
- 0008-6215
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✦ Synopsis
ABSTRACI Alkali-extractable wheat-flour arabinoxylan, treated with endo-(1 -+ 42B-o-xylanase III from Aspergilks awumori CMI 142717, was fractionated by Bio-Gel P-2 size exclusion chromatography at 60°C. Column fractions, corresponding to oligosaccharides with degrees of polymerisation from 5 to 10, were collected, and subfractionated by high performance anion-exchange chromatography on CarboPac PA-l. The structures of the oligosaccharides thus obtained were elucidated by 'H NMR spectroscopy, showing chains of (1 + 4)linked /3-D-xylopyranosyl residues differently substituted at O-3 and/or O-2,3 with cY-r.-arabinofuranosyl groups. The structures were different from those obtained with endo-(1 + 4)-/3-o-xylanase I of the same xylanolytic enzyme system.