Chaperones: Methods and Protocols

✍ Scribed by Stuart K. Calderwood,Thomas L. Prince (eds.)

• Publisher: Humana Press
• Year: 2018
• Tongue: English
• Leaves: 447
• Series: Methods in Molecular Biology 1709
• Edition: 1
• Category: Library

✦ Synopsis

This volume is a compilation of laboratory protocols and methodology required for the study of molecular chaperones and the cellular stress response. Chapters detail stress response in Hsf1, Hsf2 and Hsf4 knockout mice, mapping HSP interaction networks, the LUminescence-based Mammalian IntERactome (LUMIER), Hsp70 biology, protein folding activity of Hsp90, cytotoxicity of HSP inhibitors, computational approaches for modeling allosteric Hsp90 interactions, HSPs in immunity and vaccine development , and biologies of Hsp70 and Hsp90. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Chaperones: Methods and Protocols aims to ensure successful results in the further study of this vital field.

✦ Table of Contents

Front Matter ....Pages i-xix
Targeted Deletion of Hsf1, 2, and 4 Genes in Mice (Xiongjie Jin, Binnur Eroglu, Demetrius Moskophidis, Nahid F. Mivechi)....Pages 1-22
Role of Heat Shock Factors in Stress-Induced Transcription (Ayesha Murshid, Thomas L. Prince, Ben Lang, Stuart K. Calderwood)....Pages 23-34
Monitoring of the Heat Shock Response with a Real-Time Luciferase Reporter (Toshiki Kijima, Takanori Eguchi, Len Neckers, Thomas L. Prince)....Pages 35-45
Quantitative Profiling of Chaperone/Client Interactions with LUMIER Assay (Mikko Taipale)....Pages 47-58
Measurement of Chaperone-Mediated Effects on Polyglutamine Protein Aggregation by the Filter Trap Assay (Maria A. W. H. van Waarde-Verhagen, Harm H. Kampinga)....Pages 59-74
Fluorescent-Linked Enzyme Chemoproteomic Strategy (FLECS) for Identifying HSP70 Inhibitors (T. A. J. Haystead)....Pages 75-86
A High-Throughput Screen for Inhibitors of the Hsp90-Chaperone Machine (Jason Davenport, Lakshmi Galam, Robert L. Matts)....Pages 87-96
Primary Colorectal Cells Culture as a Translational Research Model (Sheah Lin Lee, Nina Claire Dempsey-Hibbert, Dale Vimalachandran, Terence David Wardle, Paul A. Sutton, John H. H. Williams)....Pages 97-105
Cell Death and Survival Assays (Alexander E. Kabakov, Vladimir L. Gabai)....Pages 107-127
Detecting the Potential Pharmacological Synergy of Drug Combination by Viability Assays In Vitro (Benjamin K. Gibbs, Carole Sourbier)....Pages 129-137
Proteomic Profiling of Hsp90 Inhibitors (Sudhakar Voruganti, Jake T. Kline, Maurie J. Balch, Janet Rogers, Robert L. Matts, Steven D. Hartson)....Pages 139-162
Analysis of HspB1 (Hsp27) Oligomerization and Phosphorylation Patterns and Its Interaction with Specific Client Polypeptides (André-Patrick Arrigo)....Pages 163-178
Nucleotide Exchange Factors for Hsp70 Chaperones (Heike Rampelt, Matthias P. Mayer, Bernd Bukau)....Pages 179-188
Determination of Hsp90 Activity Through Activation of Glucocorticoid Receptors in Yeast (Michael Reidy)....Pages 189-198
Bacterial Hsp90 ATPase Assays (Joel R. Hoskins, Sue Wickner, Shannon M. Doyle)....Pages 199-207
Detecting Posttranslational Modifications of Hsp90 (Rebecca A. Sager, Mark R. Woodford, Len Neckers, Mehdi Mollapour)....Pages 209-219
Chromatin Immunoprecipitation (ChIP) of Heat Shock Protein 90 (Hsp90) (Aneliya Yoveva, Ritwick Sawarkar)....Pages 221-231
A Workflow Guide to RNA-seq Analysis of Chaperone Function and Beyond (Benjamin J. Lang, Kristina M. Holton, Jianlin Gong, Stuart K. Calderwood)....Pages 233-252
Computational Modeling of the Hsp90 Interactions with Cochaperones and Small-Molecule Inhibitors (Gennady M. Verkhivker)....Pages 253-273
Computational Analysis of the Chaperone Interaction Networks (Ashwani Kumar, Kamran Rizzolo, Sandra Zilles, Mohan Babu, Walid A. Houry)....Pages 275-291
Immunohistochemistry of Human Hsp60 in Health and Disease: From Autoimmunity to Cancer (Francesco Cappello, Everly Conway de Macario, Francesca Rappa, Giovanni Zummo, Alberto J. L. Macario)....Pages 293-305
Immunohistochemical and Flow Cytometric Analysis of Intracellular and Membrane-Bound Hsp70, as a Putative Biomarker of Glioblastoma Multiforme, Using the cmHsp70.1 Monoclonal Antibody (Stefan Stangl, Gemma A. Foulds, Helena Fellinger, Geoffrey J. Pilkington, A. Graham Pockley, Gabriele Multhoff)....Pages 307-320
Detection and Analysis of Extracellular Hsp90 (eHsp90) (Stephanie Cortes, Alexander J. Baker-Williams, Mehdi Mollapour, Dimitra Bourboulia)....Pages 321-329
Molecular Chaperone Receptors (Ayesha Murshid, Jimmy Theriault, Jianlin Gong, Stuart K. Calderwood)....Pages 331-344
Creation of Recombinant Chaperone Vaccine Using Large Heat Shock Protein for Antigen-Targeted Cancer Immunotherapy (Chunqing Guo, John R. Subjeck, Xiang-Yang Wang)....Pages 345-357
A Novel Heat Shock Protein 70-based Vaccine Prepared from DC-Tumor Fusion Cells (Desheng Weng, Stuart K. Calderwood, Jianlin Gong)....Pages 359-369
Hsp70: A Cancer Target Inside and Outside the Cell (Christophe Boudesco, Sebastien Cause, Gaëtan Jego, Carmen Garrido)....Pages 371-396
Evidence for Hsp90 Co-chaperones in Regulating Hsp90 Function and Promoting Client Protein Folding (Marc B. Cox, Jill L. Johnson)....Pages 397-422
Clinical Evaluation and Biomarker Profiling of Hsp90 Inhibitors (Akira Yuno, Min-Jung Lee, Sunmin Lee, Yusuke Tomita, David Rekhtman, Brittni Moore et al.)....Pages 423-441
Back Matter ....Pages 443-445

✦ Subjects

Biochemistry