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Changing the Regioselectivity of the Tryptophan 7-Halogenase PrnA by Site-Directed Mutagenesis

✍ Scribed by Dr. Alexander Lang; Stefan Polnick; Tristan Nicke; Peter William; Dr. Eugenio P. Patallo; Prof. James H. Naismith; Prof. Karl-Heinz van Pée

Publisher
John Wiley and Sons
Year
2011
Tongue
English
Weight
443 KB
Volume
50
Category
Article
ISSN
0044-8249

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✍ Paul Holloway; Kyle L. Knoke; Jack T. Trevors; Hung Lee 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 58 KB 👁 1 views

We attempted to expand the range of chlorinated solvents degraded by Xanthobacter autotrophicus GJ10 to include trichloroethylene by the rational modification of the enzyme haloalkane dehalogenase. The amino acids Phe164, Asp170, Phe172 and Trp175 were individually replaced with alanine by site-dire