✦ LIBER ✦

Changes in type I collagen following laser welding

✍ Scribed by Dr. Lawrence S. Bass; Nader Moazami; Joanne Pocsidio; Mehmet C. Oz; Paul Logerfo; Michael R. Treat

Publisher: John Wiley and Sons
Year: 1992
Tongue: English
Weight: 559 KB
Volume: 12
Category: Article
ISSN: 0196-8092
DOI: 10.1002/lsm.1900120508

No coin nor oath required. For personal study only.

✦ Synopsis

Selection of ideal laser parameters for tissue welding is inhibited by poor understanding of the mechanism. We investigated structural changes in collagen molecules extracted from rat tail tendon (>go% type I collagen) after tissue welding using an 808 nm diode laser and indocyanine green dye applied to the weld site. Mobility patterns on SDS-PAGE were identical in the lasered and untreated tendon extracts with urea or acetic acid. Pepsin incubation after acetic acid extraction revealed a reduction of collagen alpha and beta bands in lasered compared with untreated specimens. Circular dichroism studies of rat tail tendon showed absence of helical structure in collagen from lasered tendon. No evidence for covalent bonding was present in laser-treated tissues. Collagen molecules are denatured by the laser wavelength and parameters used in this study. No significant amount of helical structure is regenerated on cooling. We conclude that noncovalent interactions between denatured collagen molecules may be responsible for the creation of tissue welding.

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