Changes in topography and function of thylakoid membranes following membrane protein phosphorylation

✍ Scribed by M. T. Black; P. Lee; P. Horton

• Book ID: 104753008
• Publisher: Springer-Verlag
• Year: 1986
• Tongue: English
• Weight: 695 KB
• Volume: 168
• Category: Article
• ISSN: 0032-0935
• DOI: 10.1007/bf00392357

✦ Synopsis

Changes in topography and function of pea (Pisum sativum L.) thylakoid membrane fractions following membrane protein phosphorylation have been studied. After protein phosphorylation the stromal membrane fraction had a higher chlorophyll a/b ratio, an increased content of light-harvesting chlorophyll protein and a higher ratio of chlorophyll to cytochrome f. This indicates that a pool of light-harvesting chlorophyll protein migrates from the photosystem II-enriched grana regions to the photosystem I-enriched stroma lamellae, in agreement with Kyle et al. (1984, Biochim. Biophys. Acta 765, 89-96) and Larsson et al. (1983, Eur. J. Biochem. 136, 25-29). Phosphorylation caused a stimulation in the rate of light-limited photosystem-I electron transfer in the unappressed membrane fraction, indicating that the translocated LHC-II becomes functionally associated with photosystem I.