Changes in three-dimensional structure of cultured S91 mouse melanoma cells associated with growth inhibition and induction of melanogenesis by retinoids
✍ Scribed by J. Lauharanta; K. Käpyaho; L. Kanerva
- Book ID
- 104764643
- Publisher
- Springer-Verlag
- Year
- 1985
- Tongue
- English
- Weight
- 974 KB
- Volume
- 277
- Category
- Article
- ISSN
- 0340-3696
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✦ Synopsis
In addition to their established use in the treatment of dermatological disorders, retinoids are potentially useful in oncology. Retinoids inhibit the proliferation of various cultured tumour cells [7], suppress the expression of the transformed phenotype [11] and enhance the differentiation of certain malignant cells [8,9,12]. $91 mouse melanoma cells are very sensitive to retinoids, which inhibit both their anchorage-dependent and anchorage-independent growth, and stimulate their melanotic expression [9]. The present paper reports the effects of two retinoids on the growth and melanogenesis of $91 melanoma cells. Using scanning electron microscopy, we found that these effects were associated with marked changes in the threedimensional structure of the cells.
Cloudman-S91 mouse melanoma cells (clone M3; Flow Laboratories, UK) were grown on plastic cellculture dishes (Sterilin; 5 cm in diameter). The culture medium was HAM' s F-10 medium supplemented with 15% horse serum, 2% foetal calf serum, 2 mM glutamine and penicilline and streptomycin (50 gg/ml each). The cells were plated at various densities (0.025-0.8 x 106/dish) and allowed to attach overnight; retinoic acid (5 gM) and etretinate (10 gM; Hoffmann-LaRoche Research Laboratories, Basel, Switzerland) were then added as ethanol solutions. The final ethanol concentration was 0.4%. Incubation was performed at 37 ~ C in an atmosphere of 5% CO2/ 95% air. The medium was changed after 3 days of retinoid treatment, and culturing was continued for 3