Pancreatic acinar cells from rats 5 to 658 days (94 weeks) of age were isolated by enzymatic dissociation and stained with the DNA specific fluorochrome Hoechst 33258. The nuclear DNA content and the incidence of binucleation were estimated in these cells. Total pancreatic weight, RNA, protein and D
Changes in the primary structure of DNA in some rat organs during aging
โ Scribed by V. K. Vasil'ev
- Publisher
- Springer US
- Year
- 1983
- Tongue
- English
- Weight
- 229 KB
- Volume
- 95
- Category
- Article
- ISSN
- 0007-4888
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We examined epinephrine-and isoproterenol-stimulated DNA synthesis in primary cultured hepatocytes from 6-, 12-, and 24-month-old rats. Epinephrinestimulated DNA synthesis in 6-month-old rat hepatocytes began after 20 h and reachcd a maximum at 50 h. Similarly, isoproterenol-stimulated DNA synthesis