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Changes in the fluorescence of bound nucleotide during the reaction catalysed by glucose-fructose oxidoreductase from Zymomonas mobilis

✍ Scribed by Michael J. HARDMAN; Marlene TSAO; Robert K. SCOPES


Book ID
115129245
Publisher
John Wiley and Sons
Year
1992
Tongue
English
Weight
622 KB
Volume
205
Category
Article
ISSN
1432-1327

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For the continuous, enzymatic synthesis of sorbitol and gluconic acid by cell-free glucose-fructose oxidoreductase (GFOR) from Zymomonas mobilis, the principal determinants of productivity have been identified. Most important, the rapid inactivation of the soluble enzyme during substrate conversion