Changes in the expression of two Epstein-Barr virus-associated antigens, EBNA and RANA, during the cell cycle of transformed human B lymphoblasts

✍ Scribed by Susan F. Slovin; John H. Vaughan; Dennis A. Carson

• Publisher: John Wiley and Sons
• Year: 1980
• Tongue: French
• Weight: 376 KB
• Volume: 26
• Category: Article
• ISSN: 0020-7136
• DOI: 10.1002/ijc.2910260103

✦ Synopsis

Two methods of cell sychronization, density-dependent arrest and double thymidine block, were used to assign two Epstein-Barr virus-associated antigens to different parts of the growth cycle of the human B lymphblastoid cell lines, WI-L2 and Raji. The Epstein-Barr nuclear antigen (EBNA), as detected by anti-complement immunofluorescence, was maximally expressed during early S phase, decreased during the G2 and M phases, and was absent in early G1. In contrast, the rheumatoid arthritis nuclear antigen (RANA), as detected by anti-immunoglobulin immunofluorescence with a prototype serum form a patient with rheumatoid arthritis, was maximally expressed during early G1 phase, progressively decreased during S and early G2, and reappeared during late G2/M.