✦ LIBER ✦

Changes in self-renewal potential of human leukemic cells (K562): A bidirectional stochastic process

✍ Scribed by Harald von Melchner; Klaus Höffken

Publisher: John Wiley and Sons
Year: 1985
Tongue: English
Weight: 691 KB
Volume: 123
Category: Article
ISSN: 0021-9541
DOI: 10.1002/jcp.1041230215

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✦ Synopsis

Abstract

Daughter cells arising from a single cell division in the leukemic cell line K562 have equivalent self‐renewal potential with respect to their ability to form clones in semisolid medium. However, individual cells isolated from these clones in sequence have vastly different abilities in their self‐renewal potentials. Thus, cells originating from a clone with any particular self‐renewal potential exhibit the full range of self‐renewal potentials–‐from highly renewing to none renewing, cells. These results show that self‐renewal potential in the K562 cell line is a random, reversible and partially noninherited characteristic. It is suggested that the stochastic variability of the intraclonal self‐renewal potential of K562 progeny cells either reflects the initial expression of a differentiation program or the expression of the predeterministic portion of the normal myelopoietic differentiation pathway.

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Rapid changes in bidirectional K+ fluxes

Rapid changes in bidirectional K+ fluxes preceding DMSO-induced granulocytic differentiation of HL-60 human leukemic cells

✍ J. J. Gargus; E. A. Adelberg; C. W. Slayman 📂 Article 📅 1984 🏛 John Wiley and Sons 🌐 English ⚖ 775 KB

## Abstract When grown in medium containing 5 mM potassium and 140 mM sodium, HL‐60, a human promyelocytic cell line, maintained a steady‐state intracellular K^+^ concentration of 145 mmol/L cells and a steady‐state intracellular Na^+^ concentration of 30 mmol/L cells. Nearly 90% of the unidirectio