Changes in rasT24 expression do not induce changes in c-jun, jun-B, or jun-D RNA levels in rat liver epithelial cells

✍ Scribed by David B. Krizman; Russell M. Lebovitz; Michael W. Lieberman

• Book ID: 102501430
• Publisher: John Wiley and Sons
• Year: 1990
• Tongue: English
• Weight: 344 KB
• Volume: 3
• Category: Article
• ISSN: 0899-1987
• DOI: 10.1002/mc.2940030505

✦ Synopsis

Abstract

We used a series of rat liver epithelial (RLE) cell lines that carry a zinc‐regulatable metallothionein/__ras__T24 fusion gene (MT__ras__T24) to investigate the relation of ras oncogene expression to steady‐state RNA levels of the jun family of genes. In these cells, steady‐state RNA levels of c‐jun, jun‐B, and jun‐D were unrelated to __ras__T24 RNA levels or the phenotypic changes induced by the ras oncogene. Steady‐state levels of the three jun mRNAs varied among different __ras__T24 transformed clones, and, although some clones exhibited concomitant induction of __ras__T24 and jun mRNAs, other clones exhibited no such correlation. We conclude that the effects of __ras__T24 in transformed RLE cells do not appear to be mediated by c‐jun, jun‐B, or jun‐D and that studies examining only a single transformed clone may give misleading results with respect to the role of various oncogenes in the transformation process.