## Changes in the brain protein levels following administration of kainic acid Kainic acid (KA), a potent neurotoxin and excitatory amino acid, leads to derangements and modulation of brain proteins. No global brain protein expression pattern induced by KA-treatment has been reported yet. We there
Changes in protein synthesis following fertilization of the mouse ovum
β Scribed by Chen, H. Y. ;Brinster, R. L. ;Merz, E. A.
- Publisher
- John Wiley and Sons
- Year
- 1980
- Tongue
- English
- Weight
- 465 KB
- Volume
- 212
- Category
- Article
- ISSN
- 0022-104X
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β¦ Synopsis
Abstract
A quantitative method utilizing double isotope labeling with ^3^Hβmethionine and ^35^Sβmethionine and twoβdimensional polyacrylamide gel electrophoresis was employed to compare synthesis of 95 individual proteins in fertilized and unfertilized mouse ova. It was found that six proteins had significantly (P < 0.05) increased rates of synthesis while 11 proteins had significantly (P < 0.05) decreased rates of synthesis following fertilization. The studies indicate that there are changes in the synthetic rates of a much larger number of proteins at the time of fertilization than expected from previous reports.
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