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Changes in protein synthesis following fertilization of the mouse ovum

✍ Scribed by Chen, H. Y. ;Brinster, R. L. ;Merz, E. A.


Publisher
John Wiley and Sons
Year
1980
Tongue
English
Weight
465 KB
Volume
212
Category
Article
ISSN
0022-104X

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✦ Synopsis


Abstract

A quantitative method utilizing double isotope labeling with ^3^H‐methionine and ^35^S‐methionine and two‐dimensional polyacrylamide gel electrophoresis was employed to compare synthesis of 95 individual proteins in fertilized and unfertilized mouse ova. It was found that six proteins had significantly (P < 0.05) increased rates of synthesis while 11 proteins had significantly (P < 0.05) decreased rates of synthesis following fertilization. The studies indicate that there are changes in the synthetic rates of a much larger number of proteins at the time of fertilization than expected from previous reports.


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