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Changes in probe sensitivity during nmr spectroscopic studies of the perfused rat heart: a warning

✍ Scribed by Jean-François Nédélec; John K. Saunders; Paul Morris; Kieran Clarke

Publisher
John Wiley and Sons
Year
1992
Tongue
English
Weight
339 KB
Volume
27
Category
Article
ISSN
0740-3194

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✦ Synopsis

Abstract

During NMR experiments on isolated, perfused rat heart, we observed a 57% change in probe sensitivity when the perfusion buffer surrounding the heart was replaced by either a low‐ionic‐strength mannitol solution or air and the heart was made ischemic. Such changes in sensitivity, should they go unrecognized, would result in substantial quantification errors.

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## Abstract Each of six perfused rat hearts was subjected to 30 min of hypoxia followed by 60 min of reoxygenation. Inversion‐recovery data on the intracellular Na NMR signal, differentiated by a shift reagent, 6 m__M__ Dy(PPP)~2~, were obtained every 5 min, and __T__~1~ values were calculated. The