Changes in intracellular cytokine levels in newborn and adult lymphocytes induced by HSV-1
✍ Scribed by Ito, Masahiro; Koide, Waka; Sakurai, Minoru
- Publisher
- John Wiley and Sons
- Year
- 1998
- Tongue
- English
- Weight
- 169 KB
- Volume
- 56
- Category
- Article
- ISSN
- 0146-6615
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✦ Synopsis
Changes in the expression of intracellular interleukin-2 (IL-2), interleukin-4 (IL-4), interferon (IFN)-␥, and tumor necrosis factor (TNF)-␣ in newborn and adult lymphocytes induced by herpes simplex virus (HSV)-1 were examined. Cord blood mononuclear cells (CBMC) or adult peripheral blood mononuclear cells (PBMC) were infected with HSV-1 and cultured with phorbol 2-myristate 13-acetate (PMA) plus ionomycin in the presence of monensin for 4 hr. Surface antigen and intracellular cytokines were stained simultaneously and analyzed by flow cytometry. The percentage of cells that expressed IL-2, IFN-␥, and TNF-␣ was significantly increased in HSV-1-infected CD3 + , CD4 + , CD8 + , CD45RA + , and CD45R0 + lymphocytes compared with uninfected lymphocytes from adult PBMC. The percentage of cells that expressed IL-2 and TNF-␣ was increased significantly in HSV-1-infected CD3 + , CD4 + , CD8 + , and CD45RA + lymphocytes compared with uninfected lymphocytes from CBMC. IFN-␥ was under the detectable level in HSV-1-infected and uninfected lymphocytes from CBMC. Intracellular IL-4 was not detected in HSV-1 or in uninfected lymphocytes from PBMC and CBMC. These results demonstrate that HSV-1 enhances intracellular levels of IL-2, IFN-␥, and TNF-␣ in adult lymphocytes and defective IFN-␥ production in cord blood.