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Changes in enzyme activities involved in the degradation of 1,3-bisphosphoglycerate during erythropoiesis in rat bone marrow

✍ Scribed by Manuel Pineda; Montserrat Pinilla; José Luque

Publisher: John Wiley and Sons
Year: 1984
Tongue: English
Weight: 338 KB
Volume: 2
Category: Article
ISSN: 0263-6484
DOI: 10.1002/cbf.290020413

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✦ Synopsis

Rat bone marrow cells have been fractionated by density gradient in Percoll. Differential counting of erythroid cells, haemoglobin concentration and bisphosphoglycerate mutase and phosphoglycerate kinase activities have been determined in cellular fractions. A s

shown by means of a statistical approach, an increase i n bisphosphoglycerate mutase activity and a slight decrease in phosphoglycerate kinase activity is found in erythroid cells as their haemoglobin content increases. Our results suggest that there is a synthesis of 2,3-bisphosphoglycerate during the erythropoietic process which parallels the synthesis of haemoglobin.

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