Changes in endogenous cell surface galactosyltransferase activity during in vitro limb bud chondrogenesis
β Scribed by Barry D. Shur; Mary Vogler; Robert A. Kosher
- Book ID
- 115891492
- Publisher
- Elsevier Science
- Year
- 1982
- Tongue
- English
- Weight
- 715 KB
- Volume
- 137
- Category
- Article
- ISSN
- 0014-4827
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Chondrogenesis was monitored in micromass cultures of mesenchymal cells derived from the distal tip of stage-25 chick limb buds over a &day period. Alcian green staining and immunofluorescent localization of cartilage-specific proteoglycans revealed the appearance of cartilage matrix by day 3 of cel
## Abstract High density limb bud micromass cultures, derived from individual fourβday embryos, were established in order to examine chondrogenesis in normal and nanomelic embryos. Oneβ and threeβday cultures revealed no morphological differences between the two genotypes. At six days in culture, t