Changes in elemental concentrations are associated with early stages of apoptosis in human monocyte-macrophages exposed to oxidized low-density lipoprotein: an X-ray microanalytical study
✍ Scribed by Skepper, Jeremy N.; Karydis, Ioannis; Garnett, Matthew R.; Hegyi, Laszlo; Hardwick, Simon J.; Warley, Alice; Mitchinson, Malcolm J.; Cary, Nat R. B.
- Publisher
- John Wiley and Sons
- Year
- 1999
- Tongue
- English
- Weight
- 213 KB
- Volume
- 188
- Category
- Article
- ISSN
- 0022-3417
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✦ Synopsis
This study examines ion homeostasis in monocyte-macrophages committed to death by apoptosis. X-ray microanalysis has been used to demonstrate that intracellular concentrations of potassium decreased whilst those of sodium increased following 3 h of exposure to 100 g/ml of oxidized low-density lipoprotein (LDL) in vitro. In contrast, the maximal incidence of cell death, as determined by the inability to exclude trypan blue, was not seen until 24 h of exposure. At 12 h, less than 1 per cent of cells were stained using terminal transferase-mediated DNA nick-end labelling, which is generally accepted as a marker of late stages in the apoptotic pathway. This is the first demonstration of early perturbations of ion homeostasis in monocyte-macrophages exposed to concentrations of oxidized LDL known to cause apoptosis.