𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Changes in DNA polymerase activity associated with cell fusion in cultures of embryonic muscle

✍ Scribed by M. O'Neill; R. C. Strohman

Publisher
John Wiley and Sons
Year
1969
Tongue
English
Weight
628 KB
Volume
73
Category
Article
ISSN
0021-9541

No coin nor oath required. For personal study only.

✦ Synopsis

In cultures of differentiating chicken embryo muscle cells there is a steep decline in DNA polymerase activity which closely parallels the time of rapid cell fusion and the formation of multinucleated myotubes. The DNA polymerase activity remaining in the cultures is almost completely associated with single unfused cells. Cell fusion does not require a confluent culture and fusion capability appears to be severely reduced i n the remaining single cells following an approximately ten hour time period during which the majority of fusion takes place. A model is presented to explain the observed kinetics of cell growth and cell fusion in vitro.

πŸ“œ SIMILAR VOLUMES

DNA copy number changes associated with
DNA copy number changes associated with characteristic LOH in islet cell carcinomas of transgenic mice
✍ Yu Ping Shi; Paul Naik; William F. Dietrich; Joe W. Gray; Douglas Hanahan; Danie πŸ“‚ Article πŸ“… 1997 πŸ› John Wiley and Sons 🌐 English βš– 125 KB

Comparative genomic hybridization (CGH) provides a method of surveying the entire tumor genome for regional variations in DNA sequence copy number. Such variations, if found recurrently, may indicate the locations of genes that contribute to tumor development through upregulation of oncogenes (copy

Reduced mitotic activity at the peripher
Reduced mitotic activity at the periphery of human embryonic stem cell colonies cultured in vitro with mitotically-inactivated murine embryonic fibroblast feeder cells
✍ Boon Chin Heng; Tong Cao; Hua Liu; Abdul Jalil Rufaihah πŸ“‚ Article πŸ“… 2005 πŸ› John Wiley and Sons 🌐 English βš– 316 KB

## Abstract This study attempted to investigate whether different levels of mitotic activity exist within different physical regions of a human embryonic stem (hES) cell colony. Incorporation of 5‐bromo‐2‐deoxyuridine (BrdU) within newly‐synthesized DNA, followed by immunocytochemical staining was

Immunophenotypic analysis of human artic
Immunophenotypic analysis of human articular chondrocytes: Changes in surface markers associated with cell expansion in monolayer culture
✍ Jose Diaz-Romero; Jean Philippe Gaillard; Shawn Patrick Grogan; Dobrila Nesic; T πŸ“‚ Article πŸ“… 2004 πŸ› John Wiley and Sons 🌐 English βš– 557 KB

## Abstract Cartilage tissue engineering relies on in vitro expansion of primary chondrocytes. Monolayer is the chosen culture model for chondrocyte expansion because in this system the proliferative capacity of chondrocytes is substantially higher compared to non‐adherent systems. However, human a

Cell cycle changes associated with forma
Cell cycle changes associated with formation of Pt-DNA adducts in human ovarian carcinoma cells with different cisplatin sensitivity
✍ Alexandra Vaisman; Maria Varchenko; Isil Said; Stephen G. Chaney πŸ“‚ Article πŸ“… 1997 πŸ› John Wiley and Sons 🌐 English βš– 291 KB πŸ‘ 1 views

The possible correlation between alterations in cytokinetic response to cisplatin (CP) treatment and drug resistance in human ovarian carcinoma cell lines was examined. Using dual parameter flow cytometry, we performed detailed time-course and dose-response analysis of cell cycle modifications in th

Neoplastic transformation of human cells
Neoplastic transformation of human cells in culture associated with deficient repair of light-induced chromosomal DNA damage
✍ Ram Parshad; Katherine K. Sanford; Gary M. Jones; Robert E. Tarone πŸ“‚ Article πŸ“… 1982 πŸ› John Wiley and Sons 🌐 French βš– 626 KB

Two lines of normal human skin fibroblasts and five derivative lines transformed in culture to neoplastic cells by chemical carcinogens were compared with respect to chromatid breakage produced by exposure to low-intensi-