Changes in concanavalin A-reactive proteins in neurological disorders

✍ Scribed by Luciano Saso; Giovanni Valentini; Maria Grazia Leone; Eleonora Grippa; Renzo Guglielmi; Luciana Paris; Gianpaolo Cantore; Bruno Silvestrini

• Publisher: John Wiley and Sons
• Year: 1999
• Tongue: English
• Weight: 172 KB
• Volume: 13
• Category: Article
• ISSN: 0887-8013
• DOI: 10.1002/(sici)1098-2825(1999)13:4<158::aid-jcla4>3.0.co;2-0

✦ Synopsis

Changes of glycosylation of cerebrospinal fluid proteins such as α 2 -macroglobulin, and prostaglandin D synthase were studied by lectin blotting, using concanavalin A, in multiple sclerosis (n = 42) and neuropathies (n = 20) in comparison to neurological controls (n = 22). The concanavalin A-reactivity of α 2macroglobulin, which was increased in the neuropathies but not in multiple sclerosis compared to controls, correlated with the total concanavalin A-reactivity in controls and neuropathies but not in multiple sclerosis, indicating that the protein could be abnormally glycosylated in the latter disease. Although the concentration and the concanavalin A-reactivity of prostaglandin D synthase were not significantly different in the three groups, the two parameters correlated only in neuropathies but not in controls or multiple sclerosis, probably due to the high heterogeneity of the protein. These changes deserve to be studied in further detail in view of their potential clinical applications.