Changes in calcium transport in mammalian sperm mitochondria and plasma membrane irradiated at 633 nm (HeNe laser)

✍ Scribed by H. Breitbart; T. Levinshal; N. Cohen; H. Friedmann; R. Lubart

• Book ID: 103994667
• Publisher: Elsevier Science
• Year: 1996
• Tongue: English
• Weight: 598 KB
• Volume: 34
• Category: Article
• ISSN: 1011-1344
• DOI: 10.1016/1011-1344(95)07281-0

✦ Synopsis

The effect of light on calcium transport in mammalian sperm mitochondria and plasma membrane was studied. Digitonine-treated spermatozoa and plasma membrane vesicles were irradiated with an HeNe laser at various powers and energy doses and Ca2+ uptake was measured by the filtration method. It was found that there is an accelerated Ca2+ uptake by the mitochondria after low power HeNe irradiation and inhibition after high power. The flux of Ca2+ from the mitochondria was also examined and was found to be unaffected by the HeNe light. The ATP-dependent Ca2+ uptake by the bovine plasma membrane vesicles was not changed by the HeNe irradiation.