Changes in Brain and Plasma Amino Acids of Mice Intoxicated with Methyl Isocyanate

✍ Scribed by Meenakshi Gupta; Vijay Prabha

• Publisher: John Wiley and Sons
• Year: 1996
• Tongue: English
• Weight: 429 KB
• Volume: 16
• Category: Article
• ISSN: 0260-437X
• DOI: 10.1002/(sici)1099-1263(199611)16:6<469::aid-jat373>3.0.co;2-q

✦ Synopsis

Precolumn derivatization with OPA was used f o r t h e analysis of brain a n d plasma free a m i n o acids of mice after the administration of different doses (0.25 ms0, 0.5 ims0 and LD,,,) of methyl isocyanate (MIC) for different durations (45 min. 4 h, 4 days and 7 days). In general, there were a dose-dependent decrease in brain free amino acid content with the exception of glycine and arginine (increased above the control level with 0.25 m5,, and 0.5 m5,, doses), and taurine which increased with 0.5 I&, and m5,, doses in 45 min. All the amino acids from plasnia were increased with all the three doses, with the exception of arginine which decreased at the 0.25 tm5" dose in 45 min. With increase in duration of observation to 4 h, 4 days and 7 days, the brain amino acid content was still below the control levels and plasma levels were higher as compared to the respective controls. The only exceptions were serine, histidine, alanine and arginine. which decreased on the 7th day. This study suggests that MIC produced an imbalance of both the brain and plasma amino acids, suggesting neurotoxic and systemic effects.