Change in GPC elution volume of polymers by adsorption onto porous glass as column packing
✍ Scribed by Kanehiro Nakamura; Ryuichi Endo
- Book ID
- 101581605
- Publisher
- John Wiley and Sons
- Year
- 1981
- Tongue
- English
- Weight
- 378 KB
- Volume
- 26
- Category
- Article
- ISSN
- 0021-8995
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✦ Synopsis
The elution volume of polystyrene or of poly(ethy1ene glycol) of various molecular weights was measured in both tetrahydrofuran and benzene at 40 or 6OoC by means of gel permeation chromatography utilizing columns packed with crosslinked polystyrene gel or porous glass. When the polystyrene gel was used as the column packing, a relationship between log [VIM and the peak elution volume for polystyrene agreed with that for poly(ethy1ene glycol). Using columns packed with glass, however, the elution volume of polystyrene was smaller than that of poly(ethy1ene glycol) at the same log [DIM. The results are considered to be attributable to the adsorption of poly(ethy1ene glycol) onto the glass. The effect of the adsorption on the elution volume was evaluated by assuming a universal calibration for columns containing porous glass; the deviation of the elution volume from the universal calibration curve increased with increase in molecular weight. At lower temperatures, even polystyrene was adsorbed onto the glass. Therefore, to reduce the adsorption of polymers on glass, it is necessary to increase the temperature or use surface treatment when porous glass is used as a GPC column packing.