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Chain termination and inhibition of mammalian poly(A) polymerase by modified ATP analogues

✍ Scribed by Lisa S. Chen; Lei Du-Cuny; Vasupradha Vethantham; David H. Hawke; James L. Manley; Shuxing Zhang; Varsha Gandhi


Book ID
113489042
Publisher
Elsevier Science
Year
2010
Tongue
English
Weight
418 KB
Volume
79
Category
Article
ISSN
0006-2952

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A novel method, a modified polymerase chain reaction (mPCR), is reported for in vitro recombination and amplification of two or more regions of DNA located in distinct parts of a genome such as exons of a gene. The reaction consists of three stages. In the first stage, the portions are amplified ind