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Cesium sulfate density gradient centrifugation of biological macromolecules in the presence of aprotic polar solvents

โœ Scribed by Hahn, C. W.

Book ID
105340878
Publisher
Wiley (John Wiley & Sons)
Year
2007
Weight
495 KB
Volume
39
Category
Article
ISSN
0449-2994

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An efficient method for the isolation of RNA from cartilage is described. The difficulties in obtaining RNA from cartilage, a tissue of low cell density and high proteoglycan content, were overcome by making several modifications to the guanidine thiocyanate/cesium chloride method of RNA extraction.