The classical method of chromatography of chlorophylls and carotenoids on an adsorbent column is somewhat time-consuming and is more suitable for separating larger quantities of pigments. Therefore many authors have tried to develop a simpler method of separating these substances on paper. With the
Centrifugally accelerated paper chromatography of chloroplast pigments
β Scribed by J.M. Anderson
- Publisher
- Elsevier Science
- Year
- 1960
- Tongue
- English
- Weight
- 549 KB
- Volume
- 4
- Category
- Article
- ISSN
- 1873-3778
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In recent publications from this lal~oratory, the technique of centrifugally accelerated paper chromatography has been clescril~ecl~~ 2~:~. The apparatus employed consists essentially of a carefully balanced, motor driven, disk-shaped head, rotating in a horizontal plane, and inclosing a circular sh
In addition to most chlorophylls and their derivatives, monovinyl and divinyl chlorophyll species were separated by high-performance liquid chromatography, using a polyethylene column and a simple elution with aqueous acetone. Peak retention and resolution of the pigment separation were greatly incr
Owing to their light-harvesting and protective functions', the chlorophylls and carotenoids of the photosynthetic membrane are of key interest in studies on thylakoid architecture2, chloroplast development3 and senescence4, adaptation to environmental conditions5 and effects of pollution-induced str