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Centrifugal field relaxation and ionic strength effects on calf thymus DNA gels

✍ Scribed by Alfred J. Richard

Publisher: Wiley (John Wiley & Sons)
Year: 1983
Tongue: English
Weight: 676 KB
Volume: 22
Category: Article
ISSN: 0006-3525
DOI: 10.1002/bip.360220314

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✦ Synopsis

Abstract

The gellike phase formed by DNA on sedimentation to the bottom of a centrifuge cell has been studied as a function of centrifuge speed and of solvent ionic strength. The swelling pressure of the gel was found to be logarithmically related to the DNA concentration and also to the ionic strength of the solvent. The concept of electrostatic persistence length was useful in interpreting the gel‐phase volume changes occurring with ionic strength.

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