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Cellulose affinity purification of fusion proteins tagged with fungal family 1 cellulose-binding domain

✍ Scribed by Naohisa Sugimoto; Kiyohiko Igarashi; Masahiro Samejima


Book ID
116829454
Publisher
Elsevier Science
Year
2012
Tongue
English
Weight
596 KB
Volume
82
Category
Article
ISSN
1046-5928

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## Abstract The choline‐binding domain (ChoBD) of the carboxy‐terminal region of the __Streptococcus pneumoniae__ amidase LYTA (C‐LYTA) presents a strong affinity for tertiary amines. We report a method for single‐step purification of proteins expressed in the methylotrophic yeast __Pichia pastoris