Cellulolytic enzymes associated with the fruit rots ofCitrus sinensiscaused byAspergillus aculeatusandBotryodiplodia theobromae

✍ Scribed by V. A. Adisa; A. O. Fajola

• Book ID: 104772984
• Publisher: Springer Netherlands
• Year: 1983
• Tongue: English
• Weight: 332 KB
• Volume: 82
• Category: Article
• ISSN: 0301-486X
• DOI: 10.1007/bf00436941

✦ Synopsis

Botryodiplodia theobromae and Aspergillus aculeatus were inoculated on carboxymethylcellulose (CMC) medium and filter papers. The hydrolysis of the CMC medium and degradation of the filter papers were observed, indicating the production of the C l and C x cellulases by the two rot pathogens. The C l and C x enzymes were also detected in filtrates of rotted orange fruits incited by the two rot pathogens.

The cellulases could not induce rot development on their own. However, when they were added to pectinases in an enzyme inoculum, the incubation period for inducing rot development was shorter; thus establishing a secondary role for the cellulases in the rot development. This secondary role of the cellulases produced by the two fungi was found to be at peak at pH 7 and a temperature range of 25 ~ 30 ~ in the two fungi.