Cellular responsiveness to stimulation in vitro: Increased responsiveness to colony stimulating factor of bone marrow colony-forming cells treated with surface-active agents and cyclic 3′5′ AMP
✍ Scribed by W. A. Fleming; T. A. McNeill
- Publisher
- John Wiley and Sons
- Year
- 1976
- Tongue
- English
- Weight
- 484 KB
- Volume
- 88
- Category
- Article
- ISSN
- 0021-9541
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✦ Synopsis
Abstract
Addition of low concentrations (10 ng/ml) of saponin or Tween 80 to stimulated cultures of normal mouse bone marrow in agar increased the number of granulocyte‐macrophage colonies which developed. Addition of cyclic AMP or dibutyryl cyclic AMP in low concentration (10~−8~ to 10~−10~ M) also enhanced colony numbers although concentrations above 10~−5~ M were inhibitory. Enhancement was found when marrow cells were pre‐treated with these agents and cultured in their absence.
The agents did not stimulate colony development in the absence of colony‐stimulating factor and enhancement of colony number occurred only in cultures containing a concentration of colony‐stimulating factor which was sub‐optimal in terms of maximum colony development.
There was no indication of increased colony‐stimulating factor production by treated marrow cells under the experimental conditions used to show colony enhancement. It was concluded that the agents caused an increased responsiveness of colony‐forming cells to colony‐stimulating factor.