Primary hepatocytes were cultured at oxygen tensions similar to those reported to be present in periportal (13% 02) and pericentral (4% 0,) regions of the liver lobules. Cellular DNA and protein content of individual hepatocytes were determined simultaneously by two-parameter (DNAlprotein) flow cyto
✦ LIBER ✦
Cellular response after mobilization of metals by diethyldithiocarbamate in rat hepatocyte cultures
✍ Scribed by Ewa Hellström-Lindahl; Agneta Oskarsson
- Publisher
- Elsevier Science
- Year
- 1990
- Tongue
- English
- Weight
- 502 KB
- Volume
- 65
- Category
- Article
- ISSN
- 0300-483X
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Cell culture studies have revealed that metabolic functions of the adult hepatocyte are related to cell density. Development of the glycogenic response to insulin under glucocorticoid control was investigated in 15-and 18-dayold fetal rat hepatocytes plated at different cell densities. After culturi