Cellular location and partial characterization of the alanine aminotransferase in ribbed mussel gill tissue

✍ Scribed by Paynter, Kennedy T. ;Ellis, Lehman L. ;Bishop, Stephen H.

• Publisher: John Wiley and Sons
• Year: 1984
• Tongue: English
• Weight: 727 KB
• Volume: 232
• Category: Article
• ISSN: 0022-104X
• DOI: 10.1002/jez.1402320107

✦ Synopsis

Differential centrifugation of ribbed mussel gill tissue homogenates and extraction of the mitochondrial fraction demonstrated that most (72%) alanine aminotransferase (AlAT) activity was mitochondrial. Subsequent characterization of the cytosolic activity demonstrated properties identical to those demonstrated by the mitochondrial enzyme. Both enzyme fractions showed little variation in V, , , with pH, had low K,' s for ketoacid substrates, and were inhibited by aminooxyacetate (AOA), L-cycloserine, and