Cellular localization of the constitutive β-glucosidase in Trichoderma viride
✍ Scribed by Shoji Usami; Kohtaro Kirimura; Masatoshi Imura; Satoshi Morikawa
- Book ID
- 113396360
- Publisher
- Elsevier Science
- Year
- 1990
- Tongue
- English
- Weight
- 290 KB
- Volume
- 70
- Category
- Article
- ISSN
- 0922-338X
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The characterization of beta-glucosidase's production and distribution in a mutant strain Trichoderma viride T 100-14 at extracellular and intracellular levels were studied in this paper. Three experiment groups were done automatically with pH controlled at 4.8 during fermentation process, with 1mg/
Optimal product,ion of B-glucosidase was obtained by incubating the culture a t 27 "C in a growth medium that had an initial p H of 5.0 and contained cellobiose. The bulk of the enzyme (70%) was present in a cell-associated state (cell debris and cytosol) while only a small portion (30%) appeared in