Cellular inheritance of a Cre-activated reporter gene to determine paneth cell longevity in the murine small intestine
✍ Scribed by Heather Ireland; Carol Houghton; Louise Howard; Douglas J. Winton
- Publisher
- John Wiley and Sons
- Year
- 2005
- Tongue
- English
- Weight
- 237 KB
- Volume
- 233
- Category
- Article
- ISSN
- 1058-8388
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✦ Synopsis
Abstract
Here, we exploit an absolute differential between stem and progeny cells in their ability to express Cre from a somatically inducible transgene to determine the longevity of intestinal Paneth cells. In the Ah__cre__ transgenic line induction of Cre recombinase allows constitutive activation of a Cre‐activated reporter in intestinal precursors but not in Paneth cells. The time taken for Paneth cells to inherit the reporter (EYFP) was measured in adult Ah__cre__/R26R‐EYFP animals. Using confocal microscopy of TOPRO‐3–stained sections, both precursors and Paneth cells were identified and subsequently scored for EYFP expression. It takes up to 57 days for Paneth cells to inherit the reporter, making them three times longer‐lived than previously indicated using nucleotide incorporation and suggesting that such determinations of cell turnover may be significant underestimates. Developmental Dynamics 233:1332–1336, 2005. © 2005 Wiley‐Liss, Inc.