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Cellular Imaging Using Total Internal Reflection Fluorescence Microscopy: Theory and Instrumentation

✍ Scribed by Toomre, D.


Book ID
111647447
Publisher
Cold Spring Harbor Laboratory Press
Year
2012
Tongue
English
Weight
607 KB
Volume
2012
Category
Article
ISSN
1940-3402

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## Abstract In modern fluorescence microscopy, lasers are a widely used source of light, both for imaging in total internal reflection and epi‐illumination modes. In wide‐field imaging, scattering of highly coherent laser light due to imperfections in the light path typically leads to nonuniform il